A Secret Weapon For attune flow cytometer
A Secret Weapon For attune flow cytometer
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Information-enriched characterization of mobile populations. Morphological information and facts from images can increase to your richness of flow cytometry facts. Such as, the determine displays an normally traditional apoptosis assay employing Annexin V and PI, including cell imaging to characterize cells in each inhabitants to reveal morphologically distinct features. These insights could not have been gained from multiplex staining on your own.
No matter if you’re a starter or a professional, examine our methods to advance your investigate and skills in flow cytometry.
Blend acoustic concentrating technologies with substantial-parameter Investigation for flexible spectral unmixing and conventional workflows.
The award-winning Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating having a higher-velocity brightfield camera for simultaneous significant throughput flow cytometry and superior resolution brightfield imaging.
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Additionally, it truly is illuminating to check out how many doublets slip by way of common singlet gates, and fantastic to validate the status of unexpected double-optimistic occasions in my Investigation."
Optimizing gating method. Even robust guide singlet gating is error-vulnerable and continues to be a subjective determination position in Nearly all flow cytometry assays. Imaging can be utilized to verify and change gates to incorporate only one cells of interest.
Dependable impression quality even at high throughput Acoustic focusing along with a higher-velocity camera Mix to impression these T cells constantly at lower or superior flow fees. Easily alter aim and digital camera options to meet experimental requirements.
The Attune NxT cytometer is definitely the trusted workhorse instrument your lab can rely on for constant and reliable performance. The Attune CytPix product features a large-velocity brightfield digital camera and AI-pushed graphic analysis computer software, with the latest updates such as machine Studying tools and an intuitive user interface, enabling Improved facts precision, accuracy, and a lot quicker insights into sample biology.
Consumers can check out celebration photographs in true-time to substantiate sample excellent, enhance protocols, and document the morphology of each cell inhabitants determined for website Investigation. Imaging capture also Added benefits from acoustic focusing, which helps to posture the cells in order that a sharp, centered image is obtained.
AI-pushed automated impression Assessment Together with the Attune Cytometric Software program translates occasion attributes into distinctive morphology parameters that can be coupled with normal fluorescence and scatter parameters to reveal extremely reproducible population adjustments and distinctive subsets.
Software note: Automated picture Examination cuts down user-to-person variability in flow cytometry gating procedures
Created-in dimensionality reduction equipment elevated resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to verify envisioned inhabitants resolution in lysed complete human blood. User can see events from Every cluster for visual confirmation of cell phenotypes.
Adaptable payment prepare from Agilent Monetary Solutions allow you to have the vital instrumentation you require for instant job, or to increase your lab.
Gating on these prolonged parameters means that you can immediately and precisely detect populations of fascination and confirm gating technique with little if any guide evaluate. The Attune software program features usage of point out-of-the-art device Understanding Investigation equipment inside an expertly created person interface, rendering it extremely simple to operate.
The Attune Xenith cytometer can be a significant-parameter spectral analyzer, furnishing both of those spectral unmixing and traditional flow abilities for comprehensive and versatile cell Assessment.
Agilent Value Promise assures you at the very least 10 years of instrument use in the day you buy. In any other case, We're going to credit score you with the residual worth of the system towards an upgraded design.
In this article, we present that by gating strategies making use of graphic-based quantitative parameters can distinguish interacting cells from coincident activities to more correctly evaluate interacting cells.